研究業績

発表論文
2021


2019
  • Sato E., Maeda Y., Sato Y., Hinata A., Gomi H., Koga D., Torii S., Watanabe T., Hosaka M.; Culture in 10% O2 enhances the production of active hormones in neuro-endocrine cells by up-regulating the expression of processing enzyme. Biochemical Journal 476, 827-842


2018
  • Torii S., Kubota C., Saito N., Kawano A., Hou N., Kobayashi M., Torii R., Hosaka M., Kitamura T., Takeuchi T., Gomi H.; The pseudophosphatase phogrin enables glucose-stimulated insulin signaling in pancreatic β-cells. J. Biol. Chem. 293, 5920-5933

  • Maeda Y., Kudo S., Tsushima K., Sato E., Kubota C., Kayamori A., Bochimoto H., Koga D., Torii S., Gomi H., Watanabe T., Hosaka M.; Impaired processing of prohormones in secretogranin III null mice causes maladaptation to an inadequate diet and stress. Endocrinology 159, 1213-1227.


2017
  • Gomi H., Osawa H., Uno R., Yasui T., Hosaka M., Torii S., Tsukie A.; Canine Salivary glands; analysis of Raw and SNARE protein expression and SNARE complex formation with diverse tissue properties. J. Histochem. Cytochem. 65, 637-653


2016
  • Nakazawa N., Sato A., Hosaka M.; TORC1 activity is partially reduced under nitrogen starvation conditions in sake yeast Kyokai no. 7, Saccharomyces cerevisiae. J Biosci. Bioeng. 121, 247-252.


2015
  • Sato S., Nakazawa M., Kihara Y., Kubo Y., Sato Y., Kikuchi T., Nonaka A., Sasaki A., Iwashita J., Murata J., Hosaka M., Kobayashi M.; Partial inhibition of differentiation associated with elevated protein levels of pluripotency factors in mouse embryonic stem cells expressing exogenous EGAM1N homeoprotein. J. Biosci. Bioeng. 120, 562-569.

  • Gomi H., Morikawa S., Shinmura N., Moki H., Yasui T., Tsukise A, Torii S., Watanabe T., Maeda Y., Hosaka M.; Expression of secretogranin III in chicken endocrine cells: its relevance to the secretory granule properties of peptide prohormone processing and bioactive amine content. J. Histochem Cytochem 63, 350-3566.

  • Yoshihara T., Hosaka M., Terata M., Ichikawa K., Murayama S., Tanaka A., Mori M., Itabashi H., Takeuchi K., Tobita S.; Intracellular and in vivo oxygen sensing using phosphorescent Ir(III) complexes with a modified acetylacetonato ligand. Anal. Chem. 87, 2710-2717.

  • Yoshihara T., Murayama S., Masuda T., Kikuchi T., Yoshida K., Hosaka M., Tobita S.; Mitochondria-targeted oxygen probes based on cationic iridium complexes with a 5-amino-1, 10-phenanthroline ligand. Journal of Photochemistry and Photobiology A: Chemistry 299 , 172–182.


2014

  • Horiuchi H., Hosaka M., Mashio H., Terata M., Ishida S., Kyusin S., Okutsu T., Takeuchi T., Hiratsuka H.; Silylation Improves the Photodynamic Activity of Tetraphenylporphyrin Derivatives In Vitro and In Vivo. Chemistry 20, 6054-6060.

  • Watanabe, T., Bochimoto, H., Koga, D., Hosaka, M., Ushiki, T.; Functional Implications of the Golgi and Microtubular Network in Gonadotropes. Mol Cell Endocrinol. 385, 88-96.


2013

  • Sato S., Morita S., Iha M., Mori Y., Sugawara S., Kasuga K., Kojima I., Ozaki N., Muraguchi H., Okano K., Iwashita J., Murata J., Hosaka M., Kobayashi M.; Intact structure of EGAM1 homeoproteins and basic amino acid residues in the common homeodomain of EGAM1 and EGAM1C contribute to their nuclear localization in mouse embryonic stem cells. J. Biosci. Bioeng. 116, 141-146

  • Tsuchiya, M., Manabe Y., Yamada, K., Furuichi, Y., Hosaka, M., Fujii, N.; Chronic exercise enhances insulin secretion ability of pancreatic islets without change in insulin content in non-diabetic rats. BBRC 430, 676-682

  • Sun, M., Watanabe, T., Bochimoto, H., Sakai, Y., Torii, S., Takeuchi, T., Hosaka, M.; Multiple sorting systems for secretory granules ensure the regulated secretion of peotide hormones. Traffic 14, 205-218


2012
  • Watanabe, T., Sakai, Y., Koga, D., Bochimoto, H., Hira, Y., Hosaka, M., Ushiki, T.; A unique ball-shapeed Golgi apparatus in the rat pituitary ganadotrope: its functional implications in relation to the arrangement of microtuble network. J. Histochem. Cytochem. 60, 588-602

  • Yoshihara, T., Yamaguchi, Y., Hosaka, M., Takeuchi, T., Tobita, S.: Ratiometric Molecular Sensor for Monitoring Oxygen Levels in Living Cells. Angew Chem Int Ed Engl. 51, 4148-4151

  • Yoshihara. Y., Kobayashi, A., Oda, S., Hosaka, M., Takeuchi, T., Tobita, S.: Iridium Complex Probes for Monitoring of Cellular Oxygen Levels and Imaging of Hypoxic Tissues. Proc. SPIE 8233, 82330A1-82330A8


2011
  • Horiuchi, H., Kameya, T., Hosaka, M., Yoshimura, K., Kyushin, S., Matsumoto, H., Okutsu, T., Takeuchi, T., Hiratsuka, H. : Silylation Enhancement of Photodynamic Activity of Tetraphenylporphyrin Derivative. J. Photochem. Photobiol. 221, 98-104.

  • Yamaguchi, R., Hosaka, M., Torii, S., Hou, N., Saito, N., Yoshimoto, Y., Imai., H., Takeuchi, T.: Cyclophilin C-associated protein regulation of phagocytic functions via NFAT activation in macrophages. Brain Res. 1397, 55-65.

  • Saito, N., Takeuchi, T., Kawano, A., Hosaka, M., Hou, N., Torii, S.: Luminal Interaction of Phogrin with Carboxypeptidase E for Effective Targeting to Secretory Granules. Traffic 12, 499-506.

2010
  • Tsuchiya, M., Hosaka, M., Moriguchi, T., Zhang, S., Suda, M., Yokota-Hashimoto, H., Shinozuka, K., Takeuchi, T. (The first two authors contributed equally to this work.): Cholesterol Biosynthesis Pathway Intermediates and Inhibitors Regulate Glucose-stimulated Insulin Secretion and Secretory Granule Formation in Pancreatic beta-Cells. Endocrinology 151, 4705-4716

  • Zhang, S., Hosaka, M., Yoshihara, T., Negishi, K., Iida, Y., Tobita, S., Takeuchi, T. (The first three authors contributed equally to this work.): Phosphorescent Light-emitting Iridium Complexes Serve as a Hypoxia-sensing Probe for Tumor Imaging in Living Animals. Cancer Research, 70, 4490-4498.

  • Hosaka M, Watanabe T: Secretogranin III: a bridge between core hormone aggregates and the secretory granule membrane. Endocrine J. 57, 275-286.

2009
  • Moriguchi, T., Hosaka, M., Yosinari, A., Ozaki, A., Takeuchi, T., Shinozuka, K.: Cholesterol Analogs Labeled with Novel Silylated Fluorescent Compounds. Chemistry Letters 38, 966-967

  • Hotta, K., Hosaka, M., Tanabe, A., Takeuchi, T. (The first two authors contributed equally to this work.): Secretogranin II Binds to Secretogranin III and Forms Secretory Granules with Orexin, Neuropeptide Y and Proopiomelanocortin. J. Endocrinol. 202, 111-121

  • Yoshihara, T., Karasawa, Y., Zhang, S., Hosaka, M., Takeuchi, T., Iida, Y., Endo, K., Imamura, T., Tobita, S.: In-Vivo Phosphorescence Imaging of Cancer Using Iridium Complexes. Proc. SPIE 7190, 71900X1-71900X9

2008
  • Han, L., Suda, M., Tsuzuki, K., Wang, R., Ohe, Y., Hirai, H., Watanabe, T., Takeuchi, T., Hosaka, M.: A large form of secretogranin III functions as a sorting receptor for chromogranin A aggregates in PC12 cells. Mol. Endocrinol. 22, 1935-1949

  • Hou, N., Torii, S., Saito, N., Hosaka, M., Takeuchi, T.: Reactive Oxygen Species-Mediated Pancreatic beta-Cell Death Is Regulated by Interactions between Stress-Activated Protein Kinases, p38 and c-Jun N-Terminal Kinase, and Mitogen-Activated Protein Kinase Phosphatases. Endocrinology. 149, 1654-1665

  • Takeuchi, T., Hosaka, M.: Sorting Mechanism of Peptide Hormones and Biogenesis Mechanism of Secretory Granules by Secretogranin III, a Cholesterol-Binding Protein, in Endocrine Cells. Curr. Diabetes Rev. 4, 31-38

2007
  • Kitahara, K., Sakai, Y., Hosaka, M., Hira, Y., Kakizaki, H., Watanabe, T.: Effects of a depot formulation of the GnRH agonist leuprorelin on the ultrastructure of male rat pituitary gonadotropes. Arch Histol Cytol. 70, 79-93.

  • Hosaka, M., Watanabe, T., Yamauchi, Y., Sakai, Y., Suda, M., Mizutani, S., Takeuchi, T., Isobe, T., Izumi, T.: A subset of p23 localized on secretory granules in pancreatic beta-cells. J. Histochem. Cytochem. 55, 235-245.

2006
  • Wang, R., Hosaka, M., Han, L., Yokota-Hashimoto, H., Suda, M., Mitsushims, D., Torii, S., Takeuchi, T.: Molecular probes for sensing the cholesterol composition of subcellular organelle membranes. Biochim. Biophys. Acta. 1761, 1169-1181

2005
  • Hosaka, M., Watanabe, T., Sakai, Y., Kato, T., Takeuchi, T.: Interaction between secretogranin III and carboxypeptidase E facilitates prohormone sorting within secretory granules. J. Cell Science 118, 4785-4795.

  • Sakai Y, Hosaka M, Hira Y, Watanabe T.: Addition of phosphotungstic acid to ethanol for dehydration improves both the ultrastructure and antigenicity of pituitary tissue embedded in LR White acrylic resin. Arch Histol Cytol. 68, 337-347.

2004

  • Aihara, M., Sugawara, K., Torii, S., Hosaka, M., Kurihara, H., Saito, N., Takeuchi, T.: Angiogenic Endothelium-specific Nestin Expression is enhanced by the First Intron of the Nestin Gene. Lab. Invest. 84, 1581-1592.

  • Sakai, Y., Hosaka, M., Yoshinaga, A., Hira, Y., Harumi, T., Watanabe, T.: Immunocytochemical localization of secretogranin III in the endocrine pancreas of male rats. Arch Histol Cytol. 67, 57-64.

  • Fujisawa, T., Kamimura, H., Hosaka, M., Torii, S., Izumi, T., Kuwano, H., Takeuchi, T.: Functional localization of proprotein-convertase furin and its substrate TGF in EGF receptor-expressing gastric chief cells. Growth Factors 22, 51-59.

  • Hosaka, M., Suda, M., Sakai, Y., Izumi, T, Watanabe, T., Takeuchi, T.: Secretogranin III binds to cholesterol in the secretory granule membrane as an adapter for chromogranin A. J. Biol. Chem. 30, 3627-3634.

2003

  • Zhang, B., Hosaka, M., Sawada, Y., Torii, S., Mizutani, S., Ogata, M., Izumi, T., Takeuchi, T.: PTHrP induces insulin expression through activation of MAP kinase-specific phosphatase-1 that dephosphorylates c-jun N-terminal kinase in pancreatic beta-cells. Diabetes 52, 2720-2730.

  • Sakai, Y., Hosaka, M., Hira, Y., Harumi, T., Ohsawa, Y., Wang, H., Takeuchi, T., Uchiyama, Y., Watanabe, T.: Immunocytochemical localization of secretogranin III in the anterior lobe of male rat pituitary glands. J. Histochem. Cytochem. 51, 227-238.

2002
  • Hosaka, M., Watanabe, T., Sakai, Y., Uchiyama, Y., Takeuchi, T.: Identification of a chromogranin A domain that mediates binding to secretogranin III and targeting to secretory granules in pituitary cells and pancreatic -Cells. Mol. Biol. Cell 13, 3388-3399.

  • Yi, Z., Yokota, H., Torii, S., Aoki, T., Hosaka, M., Zhao, S., Takata, K., Takeuchi, T., Izumi, T.: The rab27a/granuphilin complex regulates the exocytosis of insulin-containing dense-core granules. Mol. Cell. Biol. 22, 1858-1867.

2000

  • Zhang, W., Li, J.L., Hosaka, M., Janz, R., Shelton, J.M., Albright, G.M., Richardson, J.A., Südhof, T.C., Victor, R.G.: Cyclosporine A-induced hypertension involves synapsin in renal sensory nerve endings. Proc. Natl. Acad. Sci. 97, 9765-9770.
1999
  • Hosaka, M., Hammer, R.E., Südhof, T.C.: A phospho-switch controls the dynamic association of synapsins with synaptic vesicles. NEURON 24, 377-387.

  • Dulubova, I., Sugita, S., Hill, S., Hosaka, M., Fernandez, I., Südhof, T.C., Rizo, J.: A conformational switch in syntaxin during exocytosis: role of munc18. EMBO J. 18, 4372-4382.

  • Hosaka, M., Südhof, T.C.: Homo- and heterodimerization of synapsins. J. Biol. Chem. 274, 16747-16753.

1998

  • Hosaka, M., Südhof, T.C.: Synapsin III, A novel synapsin with an usual regulation by Ca2+. J. Biol. Chem. 273, 13371-13374.

  • Esser, L., Wang, C.-R., Hosaka, M., Smagulla, C.S., Südhof, T.C. Deisenhofer, J.: Synapsin I is structually Similar to ATP-utilizing enzymes. EMBO J. 17, 977-984.

1996

  • Hosaka, M., Toda, K., Takatsu, H., Watanabe. T., Banno, T., Ikehara, Y., Murakami, K., Nakayama, K.: Differential effects of Brefeldin A on intracellular localization of ADP-ribosylation factors type 1 to type 6 (ARF1-ARF6) cloned from mouse. J. Biochem. (Tokyo) 120, 813-819.
1994
  • Hosaka, M., Murakami, K., Nakayama,K.: PACE4A is a ubiquitous endoprotease that has similar but not identical substrate specificity to other Kex2-like processing endoproteases. Biomed. Res. 15, 383-390.
1993
  • Kako, K., Munekata, E., Hosaka, M., Murakami, K., Nakayama,K.: Cloning and Sequence Analysis of mouse cDNAs Encoding Preprotachykinin A and B. Biomed. Res. 14, 253-259.
  • Nakagawa, T., Hosaka, M., Torii, S., Watanabe, T., Murakami, K., Nakayama, K.: Identification and Functional Expression of a New Member of the Mammalian Kex2-like Processing Endoprotease Family: Its Striking Structural Similarity to PACE4. J. Biochem. (Tokyo) 113, 132-135.

1992

  • Nakayama, K., Watanabe, T., Nakagawa, T., Kim, W.S., Nagahama, M., Hosaka, M., Hatsuzawa, K., Kondoh-Hashiba, K., Murakami, K.: Consensus Sequence for Precursor Processing at Mono-arginil Sites; Evidence for the involvement of a Kex2-like endoprotease in precursor cleavages at both dibasic and mono-arginil sites. J. Biol. Chem. 267, 16335-16340.

  • Nakayama, K., Kim, W.S., Torii, S., Hosaka, M., Nakagawa, T., Ikemizu, J., Baba, T., Murakami, K.: Identification of the fourth member of mammalian endoprotease family homologous to the yeast Kex2 protease. J. Biol. Chem. 267, 5897-5900.

1991
  • Hosaka, M., Nagahama, M., Kim, W.S., Watanabe, T., Hatsuzawa, K., Ikemizu, J., Murakami, K., Nakayama, K.: Arg-X-Lys/Arg-Arg motif as a signal for precursor cleavage catalyzed by furin within the constitutive secretory pathway. J. Biol. Chem. 266, 12127-12130.

  • Nakayama, K., Hosaka, M., Hatsuzawa, K., Murakami, K.: Cloning functional expression of a novel endoprotease involved in prohormone processing at dibasic sites. J. Biochem. (Tokyo) 109, 803-806.

1990
  • Hatsuzawa, K., Hosaka, M., Nakagawa, T., Nagase, M., Showda, A., Murakami, K., Nakayama, K.: Structure and expression of mouse furin, a yeast-related protease; Lack of processing of coexpressed prorenin in GH4C1 cells. J. Biol. Chem. 265, 22075-22078.


日本語総説
  • 古市泰郎、山田健一朗、土屋美穂、穂坂正博、眞鍋康子、0藤井宣晴:継続的なトレーニングは膵β細胞のインスィン分泌能を高める、体力科学63(1), 93, 2014

  • 飛田成史、吉原利忠、穂坂正博、竹内利行りん光分子プローブを用いた癌環境イメージング-低酸素の可視化、Medical Science Digest 35, 538-541, 2009.

  • 渡部 剛、阪井裕子、平義樹、暮地本宙己、穂坂正博:内分泌細胞における分泌顆粒形成機構、顕微鏡 43:29-34, 2008

  • 穂坂正博: ホルモンが分泌顆粒へ選別されるメカニズム、生化学(社団法人生化学) 79, 166-170, 2007


著書
  • 北村忠弘、北川浩史、穂坂正博、小島至 著;生活習慣病最前線;上毛新聞社・ 2012年

  • 小島至、伴野祥一、竹内利行、柴田宏、穂坂正博、久保原禅、山西哲郎、樋口剛、高橋久仁子 著;群馬に多い病気(コレステロールは悪ものか?);上毛新聞社・ 2007年

  • 群馬大学教員による共著新時代への視点(コレステロールと分泌顆粒);上毛新聞社・2006年