makoba.Lab@APU

発表論文


著書

Kobayashi M., Yamauchi Y. and Tanaka A. (1998):
Antisense Rb RNA inhibits in vitro myogenic differentiation in a myoblast cell line, C2.
Animal Cell Technology: Basic & Applied Aspects, 9: 41-44 (Nagai K. & Wachi M., Eds.), Kluwer Academic Publishers, Dordrecht, The Netherlands.

林 要喜知,小林 正之(1997):
神経生物学のための遺伝子導入発現研究法 (吉川 和明 編),5. 遺伝子発現分析法,5.3. クローン化遺伝子からの蛋白質発現法,A. 哺乳動物細胞 (COS細胞) 系
ニューロサイエンスラボマニュアル,2: 289-300,吉川和明(編),スプリンガー・フェアラーク東京.


総説・解説

小林 正之(2017):
ES細胞・iPS細胞の研究動向.
宮城県獣医師会会報,70: 186-191.

小林 正之(1998):
哺乳動物初期胚の成長制御因子:マウス初期胚体外培養系を利用した新規細胞成長因子の探索.
日本比較内分泌学会ニュース,89: 19-29.


原著論文

*Corresponding author
**These authors contributed equally to this work.

Sato Suguru**, Kikuchi T.**, Nishimura Y., Yugami Y., Sakuraoka M., Kita Y., Fukuda T., and Kobayashi M.* (2020):
Generation of mouse iPS cells using an inducible expression of transgenes via the cumate gene-switch.
Analytical Biochemistry, 599: 113748.

Kumagai Y., Kikuchi T., Nonaka A., Hiraide M., Sato Suguru, Sakuraoka M., Sasaki A. and Kobayashi M.* (2019):
Site-directed mutagenesis of cysteine to serine residues affects heparin binding and mitogenicity in fibroblast growth factor 4 produced in Escherichia coli.
Biotechnology & Biotechnological Equipment
, 33: 498-503.

Mori Y., Sakuraoka M., Suzuki T., Sato Sho, Sugawara S., Hiraide M., Sato Suguru and Kobayashi M.* (2018):
Exogenous TPRX1 homeoprotein modulates the gene expression of lineage-specific transcription factors in human embryonal carcinoma cells.
Biotechnology & Biotechnological Equipment, 32: 646-652.

Sasaki A., Hiwatashi K., Kumagai K., Hata K. and Kobayashi M. (2017):
Relationships between the expression of hepatocyte nuclear factors and factors essential for lipoprotein production in a human mesenchymal stem cell line, UE7T-13.
Bioscience, Biotechnology, and Biochemistry, 81: 262-270.

Sasaki A., Kimura F., Miura M., Toshima G., Takahashi J., Maruya S., Kobayashi M. and Hata K. (2017):
Lipoprotein profiles of hepatic cell lines at various stages of differentiation.
In Vitro Cellular & Developmental Biology - Animal, 53: 93-95.

Kasuga K., Sasaki A., Matsuo T., Yamamoto C., Minato Y., Kuwahara N., Fujii C., Kobayashi M., Agematu H., Tamura T., Komatsu M., Ishikawa J., Ikeda H. and Kojima I. (2017):
Heterologous production of kasugamycin, an aminoglycoside antibiotic from Streptomyces kasugaensis, in Streptomyces lividans and Rhodococcus erythropolis L-88 by constitutive expression of the biosynthetic gene cluster.
Applied Microbiology and Biotechnology, 101: 4259-4268.

Sato Yuki, Sato Shiori, Kikuchi T., Nonaka A., Kumagai Y., Sasaki A. and Kobayashi M.* (2016):
Knockdown of gene expression by antisense morpholino oligos in preimplantation mouse embryos cultured in vitro.
Analytical Biochemistry
, 509: 41-45.

Nonaka A.**, Yoshida M.**, Iha M., Kubo Y., Kihara Y., Kikuchi T., Kumagai Y., Sasaki A. and Kobayashi M.* (2016):
Exogenous expression of homeoprotein EGAM1N prevents in vitro cardiomyogenesis by impairing expression of T and Nkx2.5, but not Mef2c, in mouse embryonic stem cells.
Cytotechnology, 68: 2431-2436.

Kikuchi T.**, Kusuhara N.**, Nonaka A., Kumagai Y., Hiraide M., Sasaki A., Fukuda T. and Kobayashi M.* (2016):
Efficient generation of mouse induced pluripotent stem-like cells using a powerful CAG promoter, the piggyBac transposon, and a lipofection method.
Tohoku Journal of Animal Science and Technology (In Japanese), 66: 16-22.

Sato Shiori**, Nakazawa M.**, Kihara Y.**, Kubo Y., Sato Yuki, Kikuchi T., Nonaka A., Sasaki A., Iwashita J., Murata J., Hosaka M. and Kobayashi M.* (2015):
Partial inhibition of differentiation associated with elevated protein levels of pluripotency factors in mouse embryonic stem cells expressing exogenous EGAM1N homeoprotein.
Journal of Bioscience and Bioengineering,
120: 562-569.

Sato Yuki, Sato Shiori, Kikuchi T., Nonaka A., Sasaki A. and Kobayashi M.* (2015):
Induction of expression of the Wnt gene family in differentiating mouse embryonic stem cells expressing exogenous EGAM1N or EGAM1C homeoproteins.
Tohoku Journal of Animal Science and Technology (In Japanese), 65: 14-21.

Leslie J., Huang S., Opp J., Nagy M., Kobayashi M., Young V., and Spence J. (2015):
Persistence and toxin production by Clostridium difficile within human intestinal organoids results in disruption of epithelial paracellular barrier function.
Infection and Immunity, 83: 138-145.

Sugawara S., Ito T., Sato Shiori, Sato Yuki, Sasaki A., Fukuda T., Yamanaka K., Sakatani M., Takahashi M. and Kobayashi M.* (2015):
Generation of aminoterminally truncated, stable types of bioactive bovine and porcine fibroblast growth factor 4 in Escherichia coli.
Biotechnology and Applied Biochemistry,
62: 164-172.

Donai K., Inagaki A., So K-H., Kuroda K., Sone H., Kobayashi M., Nishimori K. and Fukuda T. (2015):
Low-molecular-weight inhibitors of cell differentiation enable efficient growth of mouse iPS cells under feeder-free conditions.
Cytotechnology,
67: 191-197.

Tomotsune K., Kasuga K., Tsuchida M., Shimura Y., Kobayashi M., Agematsu H., Ikeda H., Ishikawa J., and Kojima I. (2014):
Cloning and sequence analysis of the cellulase genes isolated from two cellulolytic Streptomycetes and their heterologous expression in Streptomyces lividans.
International Journal of the Society of Materials Engineering for Resources, 20: 213-218.

Sugawara S., Ito T., Sato Shiori, Sato Yuki, Kasuga K., Kojima I. and Kobayashi M.* (2014):
Production of an aminoterminally truncated, stable type of bioactive mouse fibroblast growth factor 4 in Escherichia coli.
Journal of Bioscience and Bioengineering, 117: 525-530.

Sugawara S., Sato Shiori, Sato Yuki, Kasuga K., Kojima I., Fukuda T. and Kobayashi M.* (2014):
Growth-promoting activity of bacterially expressed, 6x histidine-tagged porcine fibroblast growth factor 4 (HispFGF4) in porcine-derived cells cultured in vitro: a potential mechanism.
Tohoku Journal of Animal Science and Technology (In Japanese), 63: 38-43.

Sugawara S., Ito T., Sato Shiori, Sato Yuki, Kasuga K., Kojima I. and Kobayashi M.* (2014):
Identification of site-specific degradation in bacterially expressed human fibroblast growth factor 4 and generation of an aminoterminally truncated, stable form.
Applied Biochemistry and Biotechnology, 172: 206-215.

Donai K., Kuroda K., Guo Y., So K-H., Sone H., Kobayashi M., Nishimori K. and Fukuda T. (2013):
Establishment of a reporter system to monitor silencing status in induced pluripotent stem cell lines.
Analytical Biochemistry, 443: 104-112.

Shioya T., Nakamura H., Ishii N., Takahashi N., Sakamoto Y., Ozaki N., Kobayashi M., Okano K., Kamada T. and Muraguchi H. (2013):
The Coprinopsis cinerea septin Cc.Cdc3 is involved in stipe cell elongation.
Fungal Genetics and Biology, 58-59: 80-90.

Sato Sho, Morita S., Iha M., Mori Y., Sugawara S., Kasuga K., Kojima I., Ozaki N., Muraguchi H., Okano K., Iwashita J., Murata J., Hosaka M. and Kobayashi M.* (2013):
Intact structure of EGAM1 homeoproteins and basic amino acid residues in the common homeodomain of EGAM1 and EGAM1C contribute to their nuclear localization in mouse embryonic stem cells.
Journal of Bioscience and Bioengineering, 116: 141-146.

Sugawara S., Ito T., Sato Sho, Yokoo Mari, Mori Y., Kasuga K., Kojima I., Fukuda T., Yamanaka K., Sakatani M., Takahashi M. and Kobayashi M.* (2013):
Production of bioactive bovine fibroblast growth factor 4 in E. coli based on the common nucleotide sequence of its structural gene in three breeds.
Animal Science Journal, 84: 275-280.

Sato Sho, Mori Y., Sugawara S., Sato Shiori, Sato Yuki, Kasuga K., Kojima I. and Kobayashi M.* (2013):
Nucleocytoplasmic localization of EGAM1 homeoproteins ectopically expressed in mammalian cells cultured in vitro.

Tohoku Journal of Animal Science and Technology (In Japanese), 63: 1-6.

Kasuga K., Nitta A., Kobayashi M., Habe H., Nojiri H., Yamane H., Omori T. and Kojima I. (2013):
Cloning of dfdA genes from Terrabacter sp. strain DBF63 encoding dibenzofuran 4,4a-dioxygenase and heterologous expression in Streptomyces lividans.
Applied Microbiology and Biotechnology, 97: 4485-4498.

Sugawara S., Ito T., Suzuki H., Takahashi T., Konishi J., Kobayashi Masaki, Sato Sho, Mori Y., Kasuga K., Kojima I., Fukuda T. and Kobayashi M.* (2013):
Common amino acid sequences deduced from coding exons of the porcine FGF4 gene in two breeds and production of the encoded protein in Escherichia coli.
Bioscience, Biotechnology, and Biochemistry, 77: 173-177.

Mori Y.**, Suzuki A.**, Sato Sho, Sugawara S., Kasuga K., Kojima I., Iwashita J. and Kobayashi M.* (2013):
Effects of ectopic expression of EGAM1 homeoproteins and an HDAC inhibitor sodium butyrate on the induction of endogenous gene expression in mouse fibroblast NIH3T3 cells.
Tohoku Journal of Animal Science and Technology (In Japanese), 62: 8-14.

Saito K., Abe H., Nakazawa M., Irokawa E., Kasuga K., Kojima I., Murata J. and Kobayashi M.* (2012):
Ontogenic expression patterns of transcripts encoding EGAM1 homeoproteins during murine organogenesis.
Biotechnology & Biotechnological Equipment, 26: 3321-3323.

Iha M., Soma M., Sato Sho, Mori M., Sugawara S., Kasuga K., Kojima I., Yamada S., Sakaki S. and Kobayashi M.* (2012):
Severe inhibition of in vitro cardiomyogenesis in mouse embryonic stem cells ectopically expressing EGAM1C homeoprotein.
Bioscience, Biotechnology, and Biochemistry, 76: 1410-1412.

Soma M., Iha M., Kihara Y., Sato Shiori, Sato Yuki, Sato Sho, Mori Y., Sugawara S., Kasuga K., Kojima I. and Kobayashi M.* (2012):
Preferential emergence of cell types expressing markers for primitive endoderm lineages in mouse embryonic stem cells expressing exogenous EGAM1 homeoprotein.
Journal of Bioscience and Bioengineering, 114: 342-346.

Iha M.**, Watanabe M.**, Kihara Y., Sugawara S., Saito K., Soma M., Sato Sho, Mori Y., Kasuga K., Kojima I., Sasamura R., Murata J. and Kobayashi M.* (2012):
Effect of ectopic expression of homeoprotein EGAM1C on the cell morphology, growth, and differentiation in a mouse embryonic stem cell line, MG1.19 cells.
Reproduction, 143: 477-489.

Sato Sho, Takahashi T., Nishinomiya H., Katoh M., Itoh R., Yokoo Masaki, Yokoo Mari, Iha M., Mori Y., Kasuga K., Kojima I. and Kobayashi M.* (2012):
Common nucleotide sequence of structural gene encoding fibroblast growth factor 4 in eight cattle derived from three breeds.
Animal Science Journal, 83: 260-262.

Iha M.**, Ono Y.**, Sato Sho, Mori Y., Kasuga K., Kojima I. and Kobayashi M.* (2012):
Ectopic expression of EGAM1 homeoproteins induces the expression of Oct4 or Nanog in mouse fibroblast NIH3T3 and myoblast C2C12 cells.
Tohoku Journal of Animal Science and Technology, 61: 35-40 (In Japanese).

Soma M., Iha M., Sato Sho, Mori Y., Kasuga K., Kojima I. and Kobayashi M.* (2011):
Factors affecting the expression of differentiation marker genes for the primitive endoderm lineage in a mouse extra-embryonic endoderm stem cell line, XEN26 cells.
Biotechnology & Biotechnological Equipment, 25: 2679-2682.

Hosoi Y.**, Ando Y.**, Arakawa M., Kasuga K., Kojima I. and Kobayashi M.* (2011):
Production of mouse fibroblast growth factor 4 in E. coli and its application for isolation and maintenance of mouse trophoblast stem cells in vitro.
Journal of Reproduction and Development, 57: 650-654.

Kobayashi M.*, Saito K., Tamogami S., Takashima J., Kasuga K. and Kojima I. (2011):
Identification of a 2-cell stage specific inhibitor of the cleavage of preimplantation mouse embryos synthesized by rat hepatoma cells as 5'-deoxy-5'-methylthioadenosine.
Zygote, 19:117-125.

Abe H., Nakazawa M., Kasuga K., Kojima I. and Kobayashi M.* (2011):
The CAG promoter is more active than the CEF promoter for the expression of transgenes in a mouse ES cell line E14-derived EB3 cells.
Biotechnology & Biotechnological Equipment, 25: 2301-2304.

Soma M., Saito K., Hosoi Y., Iha M., Kasuga K., Kojima I. and Kobayashi M.* (2011):
Expression levels of mRNA for EGAM1 homeodomain proteins in three kinds of stem cells established from mouse blastocysts.
Tohoku Journal of Animal Science and Technology, 60: 39-46 (In Japanese).

Saito K., Ogawa A., Toyofuku K., Hosoi Y., Soma M., Iha M., Kasuga K., Kojima I., and Kobayashi M.* (2011):
Relationships between homeoprotein EGAM1C and the expression of the placental prolactin gene family in mouse placentae and trophoblast stem cells.
Reproduction, 141: 259-268.

Hosoi Y., Soma M., Saito K., Arakawa M., Ando Y., Morita S., Otsu A., Kasuga K., Kojima I. and Kobayashi M.* (2010):
Characterization of gene expression in trophoblast stem cells and extra-embryonic endoderm cells established from mouse blastocysts.
Tohoku Journal of Animal Science and Technology, 60: 6-12 (In Japanese).

Saito K., Abe H., Nakazawa M., Irokawa E., Watanabe M., Hosoi Y., Soma M., Kasuga K., Kojima I. and Kobayashi M.* (2010):
Cloning of complementary DNAs encoding structurally related homeoproteins from preimplantation mouse embryos: their involvement in the differentiation of embryonic stem cells.
Biology of Reproduction, 82: 687-697.

Kobayashi M.*, Terawaki Y., Saito K., Kasuga K. and Kojima I. (2009):
Effect of medium conditioned with rat hepatoma BRL cells on ‘2-cell block’ of random-bred mouse embryos cultured in vitro.
Zygote, 17:169-174.

Kojima I., Kasuga K., Kobayashi M., Tsuchiya S. and Ikeno S. (2006):
The biosynthesis of kasugamycin, an antibiotic against rice blast disease, with particular reference to the involvement of rpoZ, a gene encoding RNA polymerase omega subunit.
International Journal of the Society of Materials and Engineering Resources, 14: 28-32.

Kojima I., Kasuga K., Kobayashi M., Matsuo M., Akagawa H. and Mizuno S. (2003):
Cloning system for Streptomyces kasugaensis using the hybrid melamin-synthesizing gene melE as a chromogenic marker.
Actinomycetologica, 17: 29-32.

Kojima I., Kasuga K., Kobayashi M., Fukasawa A., Mizuno S., Arisawa A. and Akagawa H. (2002):
The rpoZ gene, encoding the RNA polymerase omega subunit, is required for antibiotic production and morphological differentiation in Streptomyces kasugaensis.
Journal of Bacteriology, 184: 6417-6423.

Kobayashi M.*, Sasaki H. and Ogasawara K. (2001) :
Application of redox indicator to monitor development of mouse embryos cultured in vitro.
Journal of Reproduction and Development, 47: 113-118.

Kobayashi M.*, Yamauchi Y. and Tanaka A. (1999):
Antisense p53 RNA abrogates antisense Rb RNA-induced cell death in mouse fibroblast SV-T2 cells.
Journal of Reproduction and Development, 45: 419-424.

Kobayashi M.* and Horiuchi R. (1998):
Reuber H-35 cell-conditioned medium prevents early degeneration of bovine embryos cultured in vitro.
Journal of Reproduction and Development, 44: 421-425.

Kobayashi M.*, Yamaguchi K. and Tanaka A. (1998):
Sensitive detection of rodent Rb protein by polyclonal antibodies against a bacterially expressed mouse Rb protein.
Journal of Reproduction and Development, 44: 209-214.

Kobayashi M., Yamauchi Y. and Tanaka A. (1998):
Stable expression of antisense Rb RNA inhibits terminal differentiation of mouse myoblast C2 cells.
Experimental Cell Research, 239: 40-49.

Yamauchi Y., Tanaka A., Yamaguchi K., Kobayashi M., Shimamura S. and Hanaoka F. (1998):
Apoptosis was promoted at a nonpermissive temperature in DNA replication-defective temperature-sensitive mutants of mouse FM3A cells.
Experimental Cell Research, 238: 317-323.

Kobayashi M., Tanaka A., Hayashi Y. and Shimamura S. (1997):
The CMV enhancer stimulates expression of foreign genes from the human EF-1alpha promoter.
Analytical Biochemistry, 247: 179-181.

Kobayashi M.*, Hirako M., Minato Y., Sasaki K., Horiuchi R. and Domeki I. (1997):
Rat hepatoma Reuber H-35 cells produce factors that promote the hatching of mouse embryos cultured in vitro.
Biology of Reproduction, 56: 1041-1049.

Yamauchi Y., Kobayashi M. and Tanaka A. (1996):
Application of a dicistronic mRNA expression vector to antisense RNA expression in mammalian cells.
Antisense Nucleic Acid Drug Development, 6: 305-309.

Kobayashi M., Yamauchi Y., Tanaka A. and Shimamura S. (1996):
Improved dicistronic mRNA expression vectors for efficient selection of transfectants highly expressing foreign genes.
Biotechniques, 21: 398-402.

Kobayashi M.*, Hirako M., Minato Y., Sasaki K., Horiuchi R. and Domeki I. (1996):
Rat hepatoma Reuber H-35 cells produce a 2-cell stage-specific inhibitor of the cleavage of mouse embryos.
Biology of Reproduction, 54: 364-370.

Yamaguchi K., Kobayashi M., Yamauchi Y., Tanaka A., Miyazawa H., Izumi M. and Hanaoka F. (1995):
Temperature-sensitive mutation of DNA polymerase alpha induces growth-suppressive phenotypes involving retinoblastoma protein and cyclin D1.
Cell Structure and Function, 20: 285-291.

Kobayashi M., Yamauchi Y., Yamaguchi K. and Tanaka A. (1995):
Transient expression assay for antisense RNAs using episomal replication of plasmids: effective reduction of retinoblastoma gene (Rb-1) product by its antisense RNA complementary to 3'-untranslated region.
Antisense Research and Development, 5: 141-148.

Kobayashi M. and Horiuchi R. (1995):
Actions of 3,5,3'-tri-iodothyronine on the synthesis and secretion of major plasma proteins by a human hepatoblastoma cell line (Hep G2).
Journal of Molecular Endocrinology, 14: 227-235.

Horiuchi R., Yamauchi K., Hayashi H., Koya S., Takeuchi Y., Kato K., Kobayashi M. and Takikawa H. (1989):
Purification and characterization of 55-kDa protein with 3,5,3'-triiodo-L-thyronine-binding activity and protein disulfide-isomerase activity from beef liver membrane.
European Journal of Biochemistry, 183: 529-538.

Sorimachi K., Ieiri T., Kobayashi M., Horiuchi R. and Yasumura Y. (1989):
Inhibitory effect of dibutyryl cyclic adenosine monophosphate on the induction of alkaline phosphatase in human fetal liver cell line.
Biochimica et Biophysica Acta, 1010: 72-77.

Kobayashi M., Horiuchi R., Hachisu T. and Takikawa H. (1988):
Dualistic effects of thyroid hormone on a human hepatoma cell line:  inhibition of thyroxine-binding globulin synthesis and stimulation of alpha1-acid glycoprotein synthesis.
Endocrinology, 123: 631-640.

Tetsuka M., Fukui Y., Kobayashi M., Machiyama K., Akaike M. and Ono H. (1988):
Effects of fecundin on ovulation rate and prolificacy in Suffolk ewe during the breeding and non-breeding seasons.
Japanese Journal of Anima Reproduction, 34: 91-98.

Fukui Y., Nakagawa E., Kobayashi M. and Ono H. (1987):
Influence of rams on reproductive performance of ewes mated naturally.
Japanese Journal of Sheep Science, 24: 1-5 (In Japanese).

Fukui Y., Tsubaki M., Kobayashi M. and Ono H. (1986):
Mating behavior of ram on ewes at induced estrus during the non-breeding season.
Japanese Journal of Animal Reproduction, 32: 195-201.

Takenaka, S., Kobayashi, M., Fukui, Y. and Ono, H. (1986):
Effects of semen concentration by prefreezing centrifugation on the characteristics of frozen-thawed ram semen.
Japanese Journal of Animal Reproduction, 32: 159-164 (In Japanese).

Fukui Y., Kobayashi M., Tsubaki M., Tetsuka M., Shimoda K. and Ono H. (1986):
Comparison of two ultrasonic methods for multiple pregnancy diagnosis in sheep and indicators of multiple pregnant ewes in the blood.
Animal Reproduction Science, 11: 25-33.

Kobayashi M., Fukui Y., Tetsuka M. and Ono H. (1986):
Effects of times of PMSG injection and ram introduction on estrus incidence and lambing rate in ewes treated during the non-breeding season.
Japanese Journal of Animal Reproduction, 32: 32-35 (In Japanese).

Tetsuka M., Kobayashi M., Fukui Y. and Ono H. (1985):
Observations of ovarian activities using a laparoscope in ewes induced estrus and inseminated artificially during the non-breeding season.
Japanese Journal of Animal Reproduction, 31: 169-176 (In Japanese).

Fukui Y., Kobayashi M., Kojima M. and Ono H. (1985):
Effects of time of PMSG and fixed-timed GnRH injections on estrus incidence and fertility in physiologically different ewes pre-treated with progesterone-impregnated vaginal sponge during the non-breeding season.
Theriogenology, 24: 631-641.

Kobayashi M., Fukui Y. and Ono H. (1985):
Surveys for practical application and economical profitability of the out-of-season breeding.
Japanese Journal of Sheep Science, 22: 30-33 (In Japanese).

Fukui Y., Kobayashi M., Tetsuka M., Shimoda K. and Ono H. (1985):
Artificial insemination of frozen ram semen using a laparoscope.
Japanese Journal of Sheep Science, 22: 10-14 (In Japanese).

Fukui Y., Kano H., Kobayashi M., Tetsuka M. and Ono H. (1985):
Response to repeated-superovulation treatment in the ewe.
Japanese Journal of Animal Reproduction, 31: 155-157.

Fukui Y., Kobayashi M., Tsubaki M., Kikuchi N. and Ono H. (1985):
Regulating estrus and therapy of repeat-breeder and anestrus holstein heifers using progesterone releasing intravaginal devices (PRIDs).
Japanese Journal of Veterinary Science, 47: 943-950.

Kobayashi M., Tetsuka M., Kojima M, Alfonso N. F., Fukui Y. and Ono H. (1985):
Influence of diluents, storage temperature and duration of storage on the viability of extended ram semen.
Japanese Journal of Animal Artificial Insemination Research, 7: 33-35 (In Japanese).

Fukui Y., Kobayashi M. and Ono H. (1985):
Effects of injection time of pregnant mare's serum gonadotrophin and individual rams on fertility of ewes in a trial of out-of-season breeding.
Japanese Journal of Animal Reproduction, 31: 16-24.

Kobayashi M., Fukui Y., Tsubaki M., Imai K., Kikuchi N., Kojima M., Nakatoh H. and Ono H. (1984):
Studies on the induction on of parturition in ewes by a dexamethasone injection.
Japanese Journal of Sheep Science, 21: 26-32 (In Japanese).

Fukui Y., Kobayashi M. and Ono H. (accepted):
Single and multiple pregnancy diagnosis in sheep using an ultrasonic doppler method.
International Goats and Sheep Research.

Fukui Y., Kobayashi M., Takenaka S., Kimura T., Eida K. and Ono H. (1983):
Effects of times of PMSG injection and administration of anti-PMSG or GnRH at the induced estrus on fertility of anestrus ewes for out-of-season breeding.
Japanese Journal of Sheep Science, 20: 1-7 (In Japanese).

*Corresponding author
**These authors contributed equally to this work.

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